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fluorescent dye fluorescein isothiocyanate  (MedChemExpress)


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    MedChemExpress fluorescent dye fluorescein isothiocyanate
    Fluorescent Dye Fluorescein Isothiocyanate, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 96/100, based on 131 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorescent+isothiocyanate+fitc/FITC/pm41173888-176-8-14
    Average 96 stars, based on 131 article reviews
    fluorescent dye fluorescein isothiocyanate - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    FACS:

    Article Title: Temporal biphasic regulation of photoreceptor degeneration by microglial TREM2: A metabolic-immune nexus in retinitis pigmentosa.
    Article Snippet: The retina was dissociated with 150 μl of papain enzyme in EBSS solution and 12.5 μl of DNase (Worthington Biochemical Corporation) at 37°C for 30 min. After washing one time, cell samples were incubated with Fc blocking antibody for 10 min on ice before incubating with allophycocyanin- conjugated antiCD45 (Invitrogen) and phycoerythrin (PE)–conjugated anti- CD11b (Invitrogen) in staining buffer for 30 min on ice. .. Following rinsing with FACS buffer for one time, the cell samples were incubated with fluorescent isothiocyanate (FITC)- conjugated anti- annexin V (Abcam or MCE) and propidium iodide (PI) (Abcam or MCE) in 1× binding buffer for 10 min before analyzing with BD FACSAria III Cell Sorter (BD Bioscience). ..

    Incubation:

    Article Title: Temporal biphasic regulation of photoreceptor degeneration by microglial TREM2: A metabolic-immune nexus in retinitis pigmentosa.
    Article Snippet: The retina was dissociated with 150 μl of papain enzyme in EBSS solution and 12.5 μl of DNase (Worthington Biochemical Corporation) at 37°C for 30 min. After washing one time, cell samples were incubated with Fc blocking antibody for 10 min on ice before incubating with allophycocyanin- conjugated antiCD45 (Invitrogen) and phycoerythrin (PE)–conjugated anti- CD11b (Invitrogen) in staining buffer for 30 min on ice. .. Following rinsing with FACS buffer for one time, the cell samples were incubated with fluorescent isothiocyanate (FITC)- conjugated anti- annexin V (Abcam or MCE) and propidium iodide (PI) (Abcam or MCE) in 1× binding buffer for 10 min before analyzing with BD FACSAria III Cell Sorter (BD Bioscience). ..

    Binding Assay:

    Article Title: Temporal biphasic regulation of photoreceptor degeneration by microglial TREM2: A metabolic-immune nexus in retinitis pigmentosa.
    Article Snippet: The retina was dissociated with 150 μl of papain enzyme in EBSS solution and 12.5 μl of DNase (Worthington Biochemical Corporation) at 37°C for 30 min. After washing one time, cell samples were incubated with Fc blocking antibody for 10 min on ice before incubating with allophycocyanin- conjugated antiCD45 (Invitrogen) and phycoerythrin (PE)–conjugated anti- CD11b (Invitrogen) in staining buffer for 30 min on ice. .. Following rinsing with FACS buffer for one time, the cell samples were incubated with fluorescent isothiocyanate (FITC)- conjugated anti- annexin V (Abcam or MCE) and propidium iodide (PI) (Abcam or MCE) in 1× binding buffer for 10 min before analyzing with BD FACSAria III Cell Sorter (BD Bioscience). ..



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    NFIC inhibited the nuclear entry of IRF3(5D ) . 293 T cells were co-transfected with NFIC expression plasmid, IRF3(5D) plasmid or control plasmid (EV) using Lipofectamine 2000 transfection reagent. After 30 h, the cells were fixed with 4% paraformaldehyde and treated with 0.1% Triton X-100. The cells were then incubated with Flag antibody, pIRF3 antibody, Alexa Fluor® 647 fluorescent anti-rabbit secondary antibody or FITC fluorescent anti-mouse secondary antibody. Finally, after staining with DAPI, the cells were observed using a ZEISS laser confocal microscope. Scale bar represents 10 μm. ( B ) Count the number of IRF3(5D)-positive cells and nuclear-translocated cells in 5 random fields, respectively, and calculate the ratio of nuclear-translocated IRF3(5D) cells to total IRF3(5D)-positive cells. ( C ) MARC-145 cells were co-transfected with NFIC expression plasmid, IRF3(5D) plasmid or control plasmid (EV) using Lipofectamine 2000 transfection reagent. After 30 h, nuclear-cytoplasmic fractionation was performed to analyze the protein expression levels of IRF3(5D), Lamin B, GAPDH, Flag-NFIC. To ensure the pIRF3 antibody specifically recognizes IRF3(5D), the

    Journal: BMC Veterinary Research

    Article Title: Nuclear factor IC promoting porcine reproductive and respiratory syndrome virus (PRRSV) replication and suppressing type I interferon transcription

    doi: 10.1186/s12917-025-05230-9

    Figure Lengend Snippet: NFIC inhibited the nuclear entry of IRF3(5D ) . 293 T cells were co-transfected with NFIC expression plasmid, IRF3(5D) plasmid or control plasmid (EV) using Lipofectamine 2000 transfection reagent. After 30 h, the cells were fixed with 4% paraformaldehyde and treated with 0.1% Triton X-100. The cells were then incubated with Flag antibody, pIRF3 antibody, Alexa Fluor® 647 fluorescent anti-rabbit secondary antibody or FITC fluorescent anti-mouse secondary antibody. Finally, after staining with DAPI, the cells were observed using a ZEISS laser confocal microscope. Scale bar represents 10 μm. ( B ) Count the number of IRF3(5D)-positive cells and nuclear-translocated cells in 5 random fields, respectively, and calculate the ratio of nuclear-translocated IRF3(5D) cells to total IRF3(5D)-positive cells. ( C ) MARC-145 cells were co-transfected with NFIC expression plasmid, IRF3(5D) plasmid or control plasmid (EV) using Lipofectamine 2000 transfection reagent. After 30 h, nuclear-cytoplasmic fractionation was performed to analyze the protein expression levels of IRF3(5D), Lamin B, GAPDH, Flag-NFIC. To ensure the pIRF3 antibody specifically recognizes IRF3(5D), the "IRF3" group was transfected with control plasmid and IRF3 expression plasmid simultaneously, serving as a negative control for pIRF3 antibody detection. ( D ) The grayscale values of protein bands in Fig. 6 C were analyzed by using ImageJ, with Lamin B or GAPDH as the internal reference, to compare the fold changes of nuclear-translocated IRF3(5D) in the Flag-NFIC-overexpression group to the control group. Briefly, nuclear IRF3(5D) levels were normalized to Lamin B (value 1), and total IRF3(5D) levels were normalized to GAPDH (value 2). The nuclear translocation ratio of IRF3(5D) was calculated as value 1/value 2, and fold change was determined by comparing the Flag-NFIC-overexpression group to the control group. All experiments were repeated at least three times with similar results. ***, P < 0.01

    Article Snippet: Fluorescein Isothiocyanate (FITC) fluorescent secondary antibody, Alexa Fluor® 647 fluorescent secondary antibody and 4',6-diamidino-2-phenylindole (DAPI) were purchased from Thermo Fisher Scientific.

    Techniques: Transfection, Expressing, Plasmid Preparation, Control, Incubation, Staining, Microscopy, Fractionation, Negative Control, Over Expression, Translocation Assay